Journal: Biology of reproduction

Article Title: Transforming growth factor beta1 from endometriomas promotes fibrosis in surrounding ovarian tissues via Smad2/3 signaling.

doi: 10.1093/biolre/iox140

Figure Lengend Snippet: Figure 6. Analyzing the fibrotic effects of TGF-β1 on EdSCs in vitro. After EdSCs were exposed with TGF-β1, fibrosis-related genes including COL1A1, ACTA2, CTGF, and FN1 at 4, 12, 24, 48, and 72 h were detected by RT-qPCR (A), and fibrosis-related markers including COL1A1, ACTA2, CTGF, and MMP2 were analyzed by western blotting (B). The components’ expression of the TGF-β1/Smad signaling pathway such as SMAD2, p-SMAD2, SMAD3, and p-SMAD3 were demonstrated by western blotting analysis (C).

Article Snippet: Briefly, a series of 5-μm sections was incubated with the following antibodies: monoclonal rabbit antibodies against human TGFB1 (1:200), TGF-β1 receptor (TGFBR1; 1:500; sc-398; Santa Cruz Biotechnology), SMAD2 (1:200), SMAD3 (1:200), SMAD4 (1:200), SMAD2/3 (1:200), phospho-SMAD2 (p-SMAD2; 1:200), phospho-SMAD3 (p-SMAD3; 1:200), Collagen I (COL1A1, 1:200), connective tissue growth factor (CTGF, 1:1000), alpha-smooth muscle actin (ACTA2, 1:200), cluster of differentiation 10 (CD10, 1:200; ab126593; Abcam), and a monoclonal mouse antibody against human pan cytokeratin (KRT (pan); 1:200; ab7753; Abcam).

Techniques: In Vitro, Quantitative RT-PCR, Western Blot, Expressing

Journal: Biology of reproduction

Article Title: Transforming growth factor beta1 from endometriomas promotes fibrosis in surrounding ovarian tissues via Smad2/3 signaling.

doi: 10.1093/biolre/iox140

Figure Lengend Snippet: Figure 7. IHC detection of activated TGF-β1/Smad signaling in the cystic walls of endometriomas. (A) Representative photomicrograph of an excised endometri- oma stained with Masson’s trichrome after laparoscopic cystectomy. The red lines separated ovarian tissue and the cystic wall, while yellow lines separated the endometriotic loci and the cystic wall. Follicles were highlighted with arrows in (A) and identify ovarian tissue. The expression of TGFB1 (B) and TGFBR1 (C), and fibrosis-related factors including ACTA2 (D), COL1A1 (E), and CTGF (F), and components of the TGF-β1/Smad signaling pathway including p-SMAD2 (G), p-SMAD3 (H), SMAD2/3 (I) SMAD2 (J), SMAD3 (K), and SMAD4 (L) were demonstrated in the serial sections. (M) A comparison among the percentage of positive expression areas of different primary antibody in the cystic walls of endometriomas. Magnified photomicrographs with high-resolution view areas of the blue-square frames were shown in Supplemental Figure S4 correspondingly. (A–L: 100×).

Article Snippet: Briefly, a series of 5-μm sections was incubated with the following antibodies: monoclonal rabbit antibodies against human TGFB1 (1:200), TGF-β1 receptor (TGFBR1; 1:500; sc-398; Santa Cruz Biotechnology), SMAD2 (1:200), SMAD3 (1:200), SMAD4 (1:200), SMAD2/3 (1:200), phospho-SMAD2 (p-SMAD2; 1:200), phospho-SMAD3 (p-SMAD3; 1:200), Collagen I (COL1A1, 1:200), connective tissue growth factor (CTGF, 1:1000), alpha-smooth muscle actin (ACTA2, 1:200), cluster of differentiation 10 (CD10, 1:200; ab126593; Abcam), and a monoclonal mouse antibody against human pan cytokeratin (KRT (pan); 1:200; ab7753; Abcam).

Techniques: Staining, Expressing, Comparison

Journal: Cell reports

Article Title: Therapeutic targeting of immunometabolism reveals a critical reliance on hexokinase 2 dosage for microglial activation and Alzheimer’s progression

doi: 10.1016/j.celrep.2024.114488

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Mouse TGFBR1 Taqman probe , Thermo Fisher Scientific , Mm00436964_m1.

Techniques: Recombinant, Activity Assay, Detection Assay, Proliferation Assay, Gene Expression, Knock-In, Control, Software, Western Blot

Journal: Scientific Reports

Article Title: Axl is required for TGF-β2-induced dormancy of prostate cancer cells in the bone marrow

doi: 10.1038/srep36520

Figure Lengend Snippet: ( A ) Ki-67 negative population in PC3 cells was evaluated after 4 days of co-culture with MC3T3-E1 cells. PC3 cells were treated with a TGFBR1 specific inhibitor, LY-364947 (Cat #: 2718, Tocris Bioscience, Minneapolis, MN) for 30 minutes at 37 °C, and then co-cultured with MC3T3-E1 cells. **p < 0.01 compared to PC3 cells cultured alone without the treatment of the inhibitor, ## p < 0.01 compared to PC3 cells co-cultured with MC3T3-E1 cells without the inhibitor. ( B ) Ki-67 negative populations in DU145 cells after 4 days of co-culture with MC3T3-E1 cells. *p < 0.05**, p < 0.01 compared to DU145 cells cultured alone in the absence of the inhibitor, ## p < 0.01 compared to DU145 cells co-cultured with MC3T3-E1 cells without the inhibitor.

Article Snippet: TaqMan MGB probes (Applied Biosystems) were as follows: human-specific probes; Gas6 (Hs01090305_m1), Tgfb1(Hs00998133_m1), Tgfb2(Hs00234244_m1), TGFBR1(Hs00610318_m1), TGFBR2 (Hs00559660_m1), TGFBR3 (Hs00234257_m1), p27 (CDKN1B)(Hs00153277_m1), Axl (Hs01064445_m1), Tyro3(Hs03986773_m1), MerTK(Hs00179024_m1).

Techniques: Co-Culture Assay, Cell Culture